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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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S100A9 / MRP14 Antibody (clone PS1A9‑1) LS‑C392043
MRP14 antibody LS-C392043 is an unconjugated mouse monoclonal antibody to human MRP14 (S100A9). Validated for IF and IHC.
Catalog
Size
Price
LS-C392043-20
20 µg (0.2 mg/ml)
$255
LS-C392043-100
100 µg
$395
Description
MRP14 antibody LS-C392043 is an unconjugated mouse monoclonal antibody to human MRP14 (S100A9). Validated for IF and IHC.
Target
Human S100A9 / MRP14
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgM,k Monoclonal [PS1A9-1]
Conjugations
Unconjugated
Purification
PEG purified
Modifications
Unmodified. Also available Azide-free.
Applications
  • IHC
  • IHC - Paraffin (1 - 2 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
S100A9 / MRP14 antibody was raised against recombinant human protein.
Specificity
Human S100A9 / MRP14
Usage
S100A9, also known as Calgranulin B or MRP14, is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. [UniProt] Titering of the S100A9 antibody may be required for optimal performance. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Not suitable for frozen tissue testing.
Presentation
0.01% BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
About S100A9 / MRP14
P06702 NM_002965 NP_002956.1

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Images

Immunohistochemistry

IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1).  This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC testing of FFPE human tonsil with S100A9 antibody (clone PS1A9-1). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/16/2018
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