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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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S100A9 / MRP14 Antibody (clone 47‑8D3) IHC‑plus™ LS‑B6597
Note: This antibody replaces LS-C146637
MRP14 antibody LS-B6597 is an unconjugated mouse monoclonal antibody to human MRP14 (S100A9). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6597-50
50 µg (0.1 mg/ml)
$425

Popular S100A9 / MRP14 Products

Antibody
Species: Mouse
Applications: Western blot, ELISA
Anti-S100A9 / MRP14 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Anti-S100A9 / MRP14 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: Flow Cytometry

Product Description

MRP14 antibody LS-B6597 is an unconjugated mouse monoclonal antibody to human MRP14 (S100A9). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human S100A9 / MRP14
Synonyms
S100A9, 60B8AG, Calgranulin B, CFAG, CAGB, Calgranulin-B, Calprotectin L1H subunit, CGLB, L1Ag, MRP14, NIF, p14, Protein S100-A9, LIAG, MAC387, MRP-14
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [47-8D3]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • IHC - Frozen
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
S100A9 / MRP14 antibody was raised against human peripheral blood monocyte components. Splenocytes were fused with mouse myeloma cell line NS-1.
Specificity
Reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosisantigen. Using Western blot analysis 47-8D3 was found to detect a single protein band of 14 kDin lysates of human monocytes and granulocytes, which was demonstrated to be the calcium-bindingprotein MRP14.
Usage
Staining is strongly enhanced after digestion with trypsin.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About S100A9 / MRP14
S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. P06702 NM_002965 NP_002956.1


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Images

Immunohistochemistry

Anti-S100A9 / MRP14 antibody IHC of human tonsil, leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-S100A9 / MRP14 antibody IHC of human tonsil, leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunohistochemistry

Anti-S100A9 / MRP14 antibody IHC of human tonsil, leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-S100A9 / MRP14 antibody IHC of human tonsil, leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunohistochemistry

Anti-S100A9 / MRP14 antibody IHC of human tonsil, leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-S100A9 / MRP14 antibody IHC of human tonsil, leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunohistochemistry

Anti-S100A9 / MRP14 antibody IHC of human tonsil, leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-S100A9 / MRP14 antibody IHC of human tonsil, leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Requested From: United States
Date Requested: 11/12/2018

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