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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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S100A9 / MRP14 Antibody (aa2‑114) LS‑C407888
MRP14 antibody LS-C407888 is an unconjugated rabbit polyclonal antibody to MRP14 (S100A9) from human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C407888-100
100 µg
$355

Popular S100A9 / MRP14 Products

Antibody
Species: Mouse
Applications: Western blot, ELISA
Anti-S100A9 / MRP14 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Anti-S100A9 / MRP14 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: Flow Cytometry

Product Description

MRP14 antibody LS-C407888 is an unconjugated rabbit polyclonal antibody to MRP14 (S100A9) from human, mouse, rat and other species. Validated for ELISA, IHC and WB.

Specifications

Target
Human S100A9 / MRP14
Synonyms
S100A9, 60B8AG, Calgranulin B, CFAG, CAGB, Calgranulin-B, Calprotectin L1H subunit, CGLB, L1Ag, MRP14, NIF, p14, Protein S100-A9, LIAG, MAC387, MRP-14
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
S100A9 / MRP14 antibody was raised against e. coli-derived human S100A9 recombinant protein (Position: T2-P114). Human S100A9 shares 59.8% and 64.5% amino acid (aa) sequence identity with mouse and rat S100A9, respectively.
Epitope
aa2-114
Specificity
Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes but are absent in normal tissue macrophages and lymphocytes. Under chronic inflammatory conditions, such as psoriasis and malignant disorders, also expressed in the epidermis. Found in high concentrations at local sites of inflammation or in the serum of patients with inflammatory diseases such as rheumatoid, cystic fibrosis, inflammatory bowel disease, Crohn's disease, giant cell arteritis, cystic fibrosis, Sjogren's syndrome, systemic lupus erythematosus, and progressive systemic sclerosis. Involved in the formation and deposition of amyloids in the aging prostate known as corpora amylacea inclusions. Strongly up-regulated in many tumors, including gastric, esophageal, colon, pancreatic, bladder, ovarian, thyroid, breast and skin cancers. .
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 7.1 mM sodium phosphate, 77 mM NaCl, 2.5% BSA, 0.025% sodium azide
Reconstitution
Reconstitute with 200 µl of distilled water.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.
Restrictions
For research use only.
About S100A9 / MRP14
S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. P06702 NM_002965 NP_002956.1


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Images

Immunohistochemistry

S100A9 antibody IHC-paraffin. IHC(P): Human Appendicitis Tissue.
S100A9 antibody IHC-paraffin. IHC(P): Human Appendicitis Tissue.

Immunohistochemistry

S100A9 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
S100A9 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.

Western blot

S100A9 antibody Western blot. All lanes: Anti S100A9 at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 13 kD. Observed band size: 13 kD.
S100A9 antibody Western blot. All lanes: Anti S100A9 at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 13 kD. Observed band size: 13 kD.

Immunohistochemistry

S100A9 antibody IHC-paraffin. IHC(P): Human Appendicitis Tissue.
S100A9 antibody IHC-paraffin. IHC(P): Human Appendicitis Tissue.

Immunohistochemistry

S100A9 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
S100A9 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.

Western blot

S100A9 antibody Western blot. All lanes: Anti S100A9 at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 13 kD. Observed band size: 13 kD.
S100A9 antibody Western blot. All lanes: Anti S100A9 at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 13 kD. Observed band size: 13 kD.

Immunohistochemistry

S100A9 antibody IHC-paraffin. IHC(P): Human Appendicitis Tissue.
S100A9 antibody IHC-paraffin. IHC(P): Human Appendicitis Tissue.

Immunohistochemistry

S100A9 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
S100A9 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.

Western blot

S100A9 antibody Western blot. All lanes: Anti S100A9 at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 13 kD. Observed band size: 13 kD.
S100A9 antibody Western blot. All lanes: Anti S100A9 at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 13 kD. Observed band size: 13 kD.

Immunohistochemistry

S100A9 antibody IHC-paraffin. IHC(P): Human Appendicitis Tissue.
S100A9 antibody IHC-paraffin. IHC(P): Human Appendicitis Tissue.

Immunohistochemistry

S100A9 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
S100A9 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.

Western blot

S100A9 antibody Western blot. All lanes: Anti S100A9 at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 13 kD. Observed band size: 13 kD.
S100A9 antibody Western blot. All lanes: Anti S100A9 at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 13 kD. Observed band size: 13 kD.

Requested From: United States
Date Requested: 11/13/2018

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