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Catalog Number Size Price
LS-B6516-50 50 µl (1 mg/ml) $460 
RTN4 / Nogo Antibody - Anti-RTN4 / Nogo antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
RTN4 / Nogo Antibody - Western blot of Nogo-A and -B in A) human, B) mouse, and C) rat brain tissue lysate using antibody at 1:2000.
RTN4 / Nogo Antibody - Anti-RTN4 / Nogo antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
RTN4 / Nogo Antibody - Western blot of Nogo-A and -B in A) human, B) mouse, and C) rat brain tissue lysate using antibody at 1:2000.
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IHC‑plus™ Polyclonal Rabbit anti‑Human RTN4 / Nogo Antibody (aa14‑30, IHC, WB) LS‑B6516

IHC‑plus™ Polyclonal Rabbit anti‑Human RTN4 / Nogo Antibody (aa14‑30, IHC, WB) LS‑B6516

Note: This antibody replaces LS-C19342
Antibody:
RTN4 / Nogo Rabbit anti-Human Polyclonal (aa14-30) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B6516-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RTN4 / Nogo Rabbit anti-Human Polyclonal (aa14-30) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Nogo antibody LS-B6516 is an unconjugated rabbit polyclonal antibody to Nogo (RTN4) (aa14-30) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human RTN4 / Nogo
Synonyms
RTN4 | Human NogoA | My043 protein | Neurite outgrowth inhibitor | NOGOC | NSP-CL | Nogo protein | KIAA0886 | Reticulon 5 | Reticulon-4 | Reticulon-5 | Reticulon 4 | RTN4-B1 | RTN-X | RTN4-A | RTN4-B2 | RTN4-C | Nbla00271 | Neurite growth inhibitor 220 | NI220/250 | NOGO-A | Nogo-C | ASY | Foocen | Nbla10545 | NOGO | Nogo-B | NSP
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Pig (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A synthetic sequence corresponding to amino acids 14-30 (DSPPRPQPAFKYQFVRE) of human Nogo-B was used as the immunogen, GenBank no. AAG40878.1|AF320999_1. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Pig (100%); Bovine (94%); Mouse, Rat, Bat, Dog (88%); Opossum (82%).
Epitope
aa14-30
Specificity
This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody recognizes Nogo-A (approximately 220 kD)and Nogo-B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RTN4 / Nogo
Q9NQC3 NM_020532 NP_065393.1

Validation

RTN4 / Nogo Antibody - Anti-RTN4 / Nogo antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-RTN4 / Nogo antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-RTN4 / Nogo antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LSBio Ratings

IHC-plus™ RTN4 / Nogo Antibody (aa14-30) for IHC, WB/Western LS-B6516 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination. Sissy Kalayil, Sagar Bhogaraju, Florian Bonn, Donghyuk Shin, Yaobin Liu, Ninghai Gan, Jérôme Basquin, Paolo Grumati, Zhao-Qing Luo, Ivan Dikic. Nature. 2018 May;557:734-738.

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Requested From: United States
Date Requested: 4/27/2024