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Antibodies > RPS6KA1 / RSK1 > LS-C804400
 
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Catalog Number Size Price
LS-C804400-100 100 µl (1 mg/ml) $379 
LS-C804400-200 200 µl (1 mg/ml) $421 
RPS6KA1 / RSK1 Antibody - 1:100 staining human spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RPS6KA1 / RSK1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RPS6KA1 / RSK1 Antibody - Western blot analysis of Phospho-RSK1 p90 (T359+S363) using COLO205 whole cells lysates
RPS6KA1 / RSK1 Antibody - 1:100 staining human spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RPS6KA1 / RSK1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RPS6KA1 / RSK1 Antibody - Western blot analysis of Phospho-RSK1 p90 (T359+S363) using COLO205 whole cells lysates
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Polyclonal Rabbit anti‑Human RPS6KA1 / RSK1 Antibody (phospho‑Thr359,phospho‑Ser363, IHC, WB) LS‑C804400

Polyclonal Rabbit anti‑Human RPS6KA1 / RSK1 Antibody (phospho‑Thr359,phospho‑Ser363, IHC, WB) LS‑C804400

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Antibody:
RPS6KA1 / RSK1 Rabbit anti-Human Polyclonal (pThr359,pSer363) Antibody
Application:
IHC, ICC, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C804400-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RPS6KA1 / RSK1 Rabbit anti-Human Polyclonal (pThr359,pSer363) Antibody
Application:
IHC, ICC, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
RSK1 antibody LS-C804400 is an unconjugated rabbit polyclonal antibody to RSK1 (RPS6KA1) (pThr359,pSer363) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC, Peptide-ELISA and WB.
Target
Human RPS6KA1 / RSK1
Synonyms
RPS6KA1 | MAPKAPK-1a | p90RSK | p90-RSK 1 | Pp90RSK1 | Ribosomal S6 kinase 1 | RSK-1 | S6K-alpha 1 | MAPKAP kinase 1a | MAPKAPK1A | p90 s6 kinase | p90S6K | RSK | RSK1 | HU-1 | p90RSK1 | S6K-alpha-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Phospho-RSK1 p90 (T359+S363).
Epitope
pThr359,pSer363
Specificity
Phospho-RSK1 p90 (T359+S363) Antibody detects endogenous levels of RSK1 p90 only when phosphorylated at PT359+S363.
Applications
  • IHC
  • ICC
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPS6KA1 / RSK1
Q15418 NM_002953 NP_002944.2

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Featured Products

RPS6KA1 / RSK1 Antibody - Anti-p90 RSK1 pS732 Antibody - Western Blot. Western blot of affinity purified anti-p90 RSK1 pS732 antibody shows detection of a band ~90 kD in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL. Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.
RPS6KA1 / RSK1 Antibody (phospho‑Ser732) LS‑C154250
Species: Human, Mouse, Rat, Bovine, Dog, Horse
Applications: Western blot, ELISA
Product
RPS6KA1 / RSK1 Antibody (Biotin) LS‑C268575
Species: Human
Applications: IHC, Western blot, ELISA
RPS6KA1 / RSK1 Antibody - Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with RPS6KA1 Antibody (S732), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
RPS6KA1 / RSK1 Antibody (Ser732) LS‑C99735
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
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RPS6KA1 / RSK1 Antibody (phospho‑Thr573) LS‑C38956
Species: Human, Mouse, Rat, Hamster, Chicken, Xenopus
Applications: Western blot
RPS6KA1 / RSK1 Antibody - Western blot analysis of extracts of A431 cells.
RPS6KA1 / RSK1 Antibody (phospho‑Thr359/Ser363) LS‑C411551
Species: Human
Applications: Western blot
RPS6KA1 / RSK1 Antibody
RPS6KA1 / RSK1 Antibody (clone 10B1D7A9, 10B1D7E12) LS‑C47225
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
See all RPS6KA1 / RSK1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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