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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RPS6KA1 / RSK1 Antibody (C‑Terminus) LS‑C153862

Catalog Number Size Price
LS-C153862-100 100 µg (1.2 mg/ml) $425 
Western blot of Rabbit anti-p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysates. Lane 2: EGF stimulated HEK293T cell lysates. Load: 35 ug per lane. Primary antibody: p90 RSK1 antibody at 1:1000 for overnight at 4C. Secondary antibody: peroxidase conjugated secondary antibody and ECL. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 90 kDa, ~90 kDa for p90 RSK1. Other band(s): none.

RPS6KA1 / RSK1 Antibody (C‑Terminus) LS‑C153862

Antibody:
Rabbit Polyclonal to Human RPS6KA1 / RSK1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153862-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human RPS6KA1 / RSK1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
RSK1 antibody LS-C153862 is an unconjugated rabbit polyclonal antibody to RSK1 (RPS6KA1 ) from human, mouse, rat and other species. Validated for ELISA and WB.
Target
Human RPS6KA1 / RSK1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • ELISA
Immunogen
Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Epitope
C-Terminus
Specificity
This antibody is specific for human p90 RSK1 protein. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
Usage
Anti-p90 RSK1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kD in size corresponding to p90 RSK1 protein is expected in the appropriate cell lysate or extract.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPS6KA1 / RSK1
Q15418 NM_002953 NP_002944.2

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Featured Products

Anti-p90 RSK1 pS732 Antibody - Western Blot. Western blot of affinity purified anti-p90 RSK1 pS732 antibody shows detection of a band ~90 kD in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL. Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.
Species: Human, Mouse, Rat, Bovine, Dog, Horse
Applications: Western blot, ELISA
Western blot Image
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with RPS6KA1 Antibody (S732), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Product
Species: Human
Applications: IHC, Western blot, ELISA
Product
Species: Human, Mouse, Rat, Hamster, Chicken, Xenopus
Applications: Western blot
Immunohistochemistry Image
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Requested From: United States
Date Requested: 8/18/2019
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