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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C153862-100 100 µg (1.2 mg/ml) $425 
RPS6KA1 / RSK1 Antibody - Western blot of Rabbit anti-p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysates. Lane 2: EGF stimulated HEK293T cell lysates. Load: 35 ug per lane. Primary antibody: p90 RSK1 antibody at 1:1000 for overnight at 4C. Secondary antibody: peroxidase conjugated secondary antibody and ECL. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 90 kDa, ~90 kDa for p90 RSK1. Other band(s): none.

Polyclonal Rabbit anti‑Human RPS6KA1 / RSK1 Antibody (C‑Terminus, WB) LS‑C153862

Polyclonal Rabbit anti‑Human RPS6KA1 / RSK1 Antibody (C‑Terminus, WB) LS‑C153862

Antibody:
Rabbit Polyclonal to Human RPS6KA1 / RSK1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153862-100
100 µg
1.2 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human RPS6KA1 / RSK1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
RSK1 antibody LS-C153862 is an unconjugated rabbit polyclonal antibody to RSK1 (RPS6KA1) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Target
Human RPS6KA1 / RSK1
Synonyms
RPS6KA1 | MAPKAPK-1a | p90RSK | p90-RSK 1 | Pp90RSK1 | Ribosomal S6 kinase 1 | RSK-1 | S6K-alpha 1 | MAPKAP kinase 1a | MAPKAPK1A | p90 s6 kinase | p90S6K | RSK | RSK1 | HU-1 | p90RSK1 | S6K-alpha-1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog, Horse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • ELISA (1:5000 - 1:50000)
Immunogen
Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Epitope
C-Terminus
Specificity
This antibody is specific for human p90 RSK1 protein. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
Usage
Anti-p90 RSK1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kD in size corresponding to p90 RSK1 protein is expected in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPS6KA1 / RSK1
Q15418 NM_002953 NP_002944.2

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Requested From: United States
Date Requested: 5/30/2020