Target
Human RPS27 / Ribosomal Protein S27
Synonyms
RPS27 | 40S ribosomal protein S27 | Metallopanstimulin 1 | MPS1 | Ribosomal protein S27 | Metallopan-stimulin 1 | MPS-1 | S27
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
His6-tagged human Mps1 recombinant protein.
Specificity
This antibody recognizes the N-terminus of the ~90kD human Mps1 kinase. Reactivity was confirmed with nocodazole treated HeLa cell lysates.
Applications
- Immunofluorescence (5 - 10 µg/ml)
- Western blot (1 - 3 µg/ml)
- Immunoprecipitation
- ELISA (0.1 - 1 µg/ml)
Usage
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and ELISA. Immunofluorescence: 5-10 ug/ml. Immunoprecipitation: 5-10 ug/ IP reaction. Western Blot: 1-3 ug/ml. ELISA: 0.1-1 ug/ml. Note: For immunofluorescent assays, it is recommended that cells are fixed in 4% paraformaldehyde in PBS and permeabilized with 0.2% Triton X-100. Methanol and acetone fixatives can also be used.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPS27 / Ribosomal Protein S27