(tested or 100% immunogen sequence identity)
- ICC (1:50)
- Western blot (1:500 - 1:2000)
RHOE / RND3 antibody was raised against full length fusion protein corresponding to residues 1-244 of mouse RhoE/Rnd3.
Recognizes RhoE/Rnd3, Mr ~29kD. Weakly cross-reacts with Rnd1, Mr ~27kD. Species cross-reactivity: Mouse and human.
Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 1:500-1:2000. Detected RhoE/Rnd3 in RIPA lysates from 3T3/A31 cells. Immunocytochemistry: 1:50. Has been reported by an independent laboratory to show positive immunostaining for RhoE/Rnd3 in 3T3/A31 cells fixed with paraformaldehyde and permeabilized by Triton X-100.
0.05% Sodium Azide, 30% Glycerol
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.