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Catalog Number Size Price
LS-C821425-0.2 0.2 mg (1 mg/ml) $540 
Antibody

Recombinant Monoclonal Rabbit anti‑Bovine Rhodopsin / RHO Antibody (clone Rho 1D4, IHC, IF, WB) LS‑C821425

Recombinant Monoclonal Rabbit anti‑Bovine Rhodopsin / RHO Antibody (clone Rho 1D4, IHC, IF, WB) LS‑C821425

Antibody:
Rhodopsin / RHO Rabbit anti-Bovine Recombinant Monoclonal (Rho 1D4) Antibody
Application:
IHC, IF, WB, IP, ELISA, RIA
Reactivity:
Bovine, Human, Mouse, Rat, Zebrafish, Amphibian, Cow
Format:
Unconjugated, Unmodified
Price
Catalog Number
$540
LS-C821425-0.2
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Rhodopsin / RHO Rabbit anti-Bovine Recombinant Monoclonal (Rho 1D4) Antibody
Application:
IHC, IF, WB, IP, ELISA, RIA
Reactivity:
Bovine, Human, Mouse, Rat, Zebrafish, Amphibian, Cow
Format:
Unconjugated, Unmodified

Specifications

Description
This Rhodopsin / RHO antibody is an unconjugated Rabbit/Human chimeric recombinant monoclonal antibody. Validated for ELISA, IF, IHC, IP, RIA, and WB.
Target
Bovine Rhodopsin / RHO
Synonyms
RHO | CSNBAD1 | OPN2 | Rhodopsin | RP4 | Opsin 2, rod pigment | Opsin-2
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat, Zebrafish, Amphibian, Cow (tested or 100% immunogen sequence identity)
Clonality
IgG,k Recombinant Monoclonal
Clone
Rho 1D4
Conjugations
Unconjugated
Purification
Protein A affinity purified
Modifications
Unmodified
Immunogen
Bleached bovine rod outer segment (ROS).
Specificity
The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, 0.02% Proclin 300
Storage
Store undiluted at 4°C for up to 3 months. Aliquot and store at -20°C for long term storage.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Rhodopsin / RHO
P08100 NM_000539 NP_000530.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024