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Catalog Number Size Price
LS-C667957-50 50 µl (1 mg/ml) $286 
LS-C667957-100 100 µl (1 mg/ml) $321 
REG3A Antibody
REG3A Antibody
REG3A Antibody
REG3A Antibody
REG3A Antibody
REG3A Antibody
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Polyclonal Rabbit anti‑Human REG3A Antibody (IHC, IF, WB) LS‑C667957

Polyclonal Rabbit anti‑Human REG3A Antibody (IHC, IF, WB) LS‑C667957

Antibody:
REG3A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C667957-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
REG3A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
REG3A antibody LS-C667957 is an unconjugated rabbit polyclonal antibody to human REG3A. Validated for ICC, IF, IHC and WB.
Target
Human REG3A
Synonyms
REG3A | INGAP | HIP | PAP homologous protein | PAP1 | REG-III | REG3 | PAP-H | Reg III-alpha | REG-3-alpha | Human proislet peptide | PBCGF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Modifications
Unmodified
Immunogen
Recombinant full length protein of human REG3 alpha
Specificity
Recognizes endogenous levels of REG3 alpha protein.
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin
  • ICC
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western blot analysis of REG3 alpha expression in Raji (A), SW480 (B), mouse brain (C), rat kidney (D) whole cell lysates. Immunohistochemical analysis of REG3 alpha staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of REG3 alpha staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Presentation
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About REG3A
Q06141 NM_002580 NP_002571.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/14/2024