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Catalog Number Size Price
LS-C408028-10 10 µg $318 
LS-C408028-100 100 µg $470 
RAP1A Antibody - Western blot analysis of RAP1A using anti-RAP1A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat kidney tissue lysates. Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAP1A at approximately 21KD. The expected band size for RAP1A is at 21KD.
RAP1A Antibody - RAP1A antibody Western blot. All lanes: Anti RAP1A at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: Rat Kidney Tissue Lysate at 50 ug. Lane 4: MCF-7 Whole Cell Lysate at 40 ug. Lane 5: SW620 Whole Cell Lysate at 40 ug. Predicted band size: 21 kD. Observed band size: 21 kD.
RAP1A Antibody - Western blot analysis of RAP1A using anti-RAP1A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat kidney tissue lysates. Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAP1A at approximately 21KD. The expected band size for RAP1A is at 21KD.
RAP1A Antibody - RAP1A antibody Western blot. All lanes: Anti RAP1A at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: Rat Kidney Tissue Lysate at 50 ug. Lane 4: MCF-7 Whole Cell Lysate at 40 ug. Lane 5: SW620 Whole Cell Lysate at 40 ug. Predicted band size: 21 kD. Observed band size: 21 kD.
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Polyclonal Rabbit anti‑Human RAP1A Antibody (aa156‑181, WB) LS‑C408028

Polyclonal Rabbit anti‑Human RAP1A Antibody (aa156‑181, WB) LS‑C408028

Antibody:
RAP1A Rabbit anti-Human Polyclonal (aa156-181) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C408028-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RAP1A Rabbit anti-Human Polyclonal (aa156-181) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
RAP1A antibody LS-C408028 is an unconjugated rabbit polyclonal antibody to RAP1A (aa156-181) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
Target
Human RAP1A
Synonyms
RAP1A | C21KG | G-22K | GTP-binding protein smg p21A | KREV-1 | KREV1 | RAP1 | RAS-related protein RAP1A | Ras-related protein Rap-1A | Ras-related protein Krev-1 | SMGP21
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human RAP1A (156-181 aa EIFYDLVRQINRKTPVEKKKPKKKSC), identical to the related mouse and rat sequences.
Epitope
aa156-181
Applications
  • Western blot (0.1 - 0.5 µg/ml)
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAP1A
P62834 NM_002884 NP_002875.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 5/21/2024