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Catalog Number Size Price
LS-C817087-100 100 µl $412 
RALY Antibody - Immunofluorescence staining of RALY in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RALY polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus.
RALY Antibody - Anti-RALY rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 37 kDa.
RALY Antibody - RALY was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-RALY rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RALY rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 40 kDa.
RALY Antibody - Immunofluorescence staining of RALY in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RALY polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus.
RALY Antibody - Anti-RALY rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 37 kDa.
RALY Antibody - RALY was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-RALY rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RALY rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 40 kDa.
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Polyclonal Rabbit anti‑Human RALY Antibody (IF, WB) LS‑C817087

Polyclonal Rabbit anti‑Human RALY Antibody (IF, WB) LS‑C817087

Antibody:
RALY Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$412
LS-C817087-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
RALY Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RALY antibody LS-C817087 is an unconjugated rabbit polyclonal antibody to human RALY. Validated for ICC, IF, IP and WB.
Target
Human RALY
Synonyms
RALY | Autoantigen p542 | HNRPCL2 | HnRNP core protein C-like 2 | RNA-binding protein Raly | p542
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human RALY fragment
Specificity
Human RALY
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RALY
Q9UKM9 NM_016732 NP_057951.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/29/2024