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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C153951-500 500 µg (1 mg/ml) $235 
Sheep IgG Antibody - ELISA results of purified Rabbit anti-Sheep IgG biotin conjugated antibody Min X human serum tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 µg of sheep IgG (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate 1:10, 000, and TMB-1000 substrate.

Polyclonal Rabbit Anti‑Sheep IgG Secondary Antibody (Biotin, IHC, WB) LS‑C153951

Polyclonal Rabbit Anti‑Sheep IgG Secondary Antibody (Biotin, IHC, WB) LS‑C153951

Antibody:
Sheep IgG Rabbit anti-Sheep Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Sheep
Format:
Biotin, Unmodified
Price
Catalog Number
$235
LS-C153951-500
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Sheep IgG Rabbit anti-Sheep Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Sheep
Format:
Biotin, Unmodified

Specifications

Description
Sheep IgG antibody LS-C153951 is a biotin-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Host
Rabbit
Reactivity
Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Sheep IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 9/25/2020