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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C346937-1 1 ml $395 
Antibody

Rabbit Anti‑Human IgM Fc Secondary Antibody (FITC) LS‑C346937

Rabbit Anti‑Human IgM Fc Secondary Antibody (FITC) LS‑C346937

Antibody:
Rabbit Polyclonal to Human Human IgM Fc
Application:
IHC, IHC-Fr, ICC, IF, ELISA, ID
Reactivity:
Human
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$395 
LS-C346937-1
1 ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Human IgM Fc
Application:
IHC, IHC-Fr, ICC, IF, ELISA, ID
Reactivity:
Human
Format:
FITC, Unmodified
Other formats:

Specifications

Description
Human IgM Fc antibody LS-C346937 is an FITC-conjugated rabbit polyclonal antibody. Validated for ELISA, ICC, ID, IF and IHC.
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
FITC. Also available Unconjugated or conjugated with TRITC.
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Immunofluorescence
  • ELISA
  • Immunodiffusion
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified IgM isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Human IgM, Fc specific.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against human serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgM used as immunogens. It does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in human serum. In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of human origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/21/2019