Horse IgG

Rabbit

Horse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with FITC, Biotin, HRP.

Antiserum

Unmodified

Purified normal IgG isolated from pooled horse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Horse IgG, heavy and light chains.

• Immunodiffusion

The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses IgG1 and IgG2. Reactivity with other subclasses of horse IgG has not been tested. The antiserum reacts with both Fc and Fab portions of polyclonal IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against horse serum usually a single characteristic precipitin line is observed, representing primarily IgG2. If the level of IgG1, also known as IgG(T), in the serum sample approaches the level of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in horse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG. as secondary antibody to precipitate the immunoglobulin in normal horse serum. to prepare an immunoadsorbent for the production of Ig-free horse serum, plasma or non-Ig preparations. It has not been tested for use in nephelometry, ELISA or immunohistology, but this does not exclude such use if proper controls are included.

Delipidated, heat-inactivated, lyophilized antiserum. No preservative.

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.