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Rabbit Anti-Goat IgG Secondary Antibody (Atto 532) LS-C153889

Ordering

Wt. Vol. Conc. Price
500 µg 500 µl - $375
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Goat IgG Antibodies

Rabbit Anti-Goat IgG Secondary Antibody (HRP) LS-C56294
Rabbit Polyclonal to Goat IgG
Goat
IHC - Frozen, Western blot, ELISA
HRP Conjugated
Rabbit Anti-Goat IgG Secondary Antibody (FITC) LS-C60335
Rabbit Polyclonal to Goat IgG
Goat
Immunofluorescence, Fluorophore-Linked Immunosorbent Assay
FITC Conjugated
Western blot Image
Rabbit Anti-Goat IgG Secondary Antibody (HRP) LS-C60340
Rabbit Polyclonal to Goat IgG
Goat
IHC, Western blot, ELISA
HRP Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Goat IgG
Goat
Immunofluorescence, Western blot, ELISA
Atto 532 Conjugated

Details

Goat IgG
Rabbit
Goat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Atto 532
Immunoaffinity purified
Unmodified

Applications

  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • ELISA (1:20000)

Specificity and Use

Goat IgG antibody was raised against goat IgG whole molecule.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human, Mouse or Rabbit Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% sodium azide
500 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Goat IgG

Representing approximately 75% of serum antibodies, IgG is the most common type of antibody found in the circulation. IgG molecules are created and released by plasma B cells. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds.

Western blot

Western blot
Western Blot of Atto 532 conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Atto 532 rabbit secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 25 kDa for Goat IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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