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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C748440-20 20 µl $215 
LS-C748440-50 50 µl $235 
LS-C748440-100 100 µl $295 
LS-C748440-200 200 µl $385 
PUM1 Antibody - Immunofluorescence analysis of U2OS cells using pum1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
PUM1 Antibody - Western blot analysis of extracts of various cell lines, using pum1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
PUM1 Antibody - Immunofluorescence analysis of U2OS cells using pum1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
PUM1 Antibody - Western blot analysis of extracts of various cell lines, using pum1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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Polyclonal Rabbit anti‑Human PUM1 Antibody (IF, WB) LS‑C748440

Polyclonal Rabbit anti‑Human PUM1 Antibody (IF, WB) LS‑C748440

Antibody:
Rabbit Polyclonal to Human PUM1
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$215 
LS-C748440-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human PUM1
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PUM1 antibody LS-C748440 is an unconjugated rabbit polyclonal antibody to PUM1 from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human PUM1
Synonyms
PUM1 | KIAA0099 | PUML1 | Pumilio homolog 1 | Pumilio homolog 1 (Drosophila) | HSPUM | PUMH | PUMH1 | Pumilio (Drosophila) homolog 1 | Pumilio-1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human pum1 (NP_055491.1). MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNH
Usage
The predicted MW is 119kDa/124kDa/126kDa, while the observed MW by Western blot was 126kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PUM1
Q14671 NM_014676 NP_055491.1

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Requested From: United States
Date Requested: 7/13/2020