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PUM1 Antibody LS‑C748440

Catalog Number Size Price
LS-C748440-50 50 µl $235 
LS-C748440-100 100 µl $295 
LS-C748440-200 200 µl $385 
Immunofluorescence analysis of U2OS cells using pum1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using pum1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
Immunofluorescence analysis of U2OS cells using pum1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using pum1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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PUM1 Antibody LS‑C748440

Antibody:
Rabbit Polyclonal to Human PUM1
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C748440-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PUM1
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PUM1 antibody LS-C748440 is an unconjugated rabbit polyclonal antibody to PUM1 from human and mouse. Validated for IF and WB.
Target
Human PUM1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human pum1 (NP_055491.1). MSVACVLKRKAVLWQDSFSPHLKHHPQEPANPNMPVVLTSGTGSQAQPQPAANQALAAGTHSSPVPGSIGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNH
Usage
The predicted MW is 119kDa/124kDa/126kDa, while the observed MW by Western blot was 126kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PUM1
Q14671 NM_014676 NP_055491.1

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Requested From: United States
Date Requested: 9/17/2019