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Catalog Number Size Price
LS-C662657-10 10 µg $318 
LS-C662657-100 100 µg $470 
PTPN22 / PEP Antibody - Immunohistochemistry - Anti-PTPN22/Lyp Picoband antibody
PTPN22 / PEP Antibody - Immunohistochemistry - Anti-PTPN22/Lyp Picoband antibody
PTPN22 / PEP Antibody - Western blot analysis of PTPN22 using anti-PTPN22 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW579 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human Hela whole cell lysates, Lane 5: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN22 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTPN22 at approximately 92KD. The expected band size for PTPN22 is at 92KD.
PTPN22 / PEP Antibody - Western blot - Anti-PTPN22/Lyp Picoband antibody
PTPN22 / PEP Antibody - Immunohistochemistry - Anti-PTPN22/Lyp Picoband antibody
PTPN22 / PEP Antibody - Immunohistochemistry - Anti-PTPN22/Lyp Picoband antibody
PTPN22 / PEP Antibody - Western blot analysis of PTPN22 using anti-PTPN22 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW579 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human Hela whole cell lysates, Lane 5: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN22 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTPN22 at approximately 92KD. The expected band size for PTPN22 is at 92KD.
PTPN22 / PEP Antibody - Western blot - Anti-PTPN22/Lyp Picoband antibody
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Polyclonal Rabbit anti‑Mouse PTPN22 / PEP Antibody (IHC, WB) LS‑C662657

Polyclonal Rabbit anti‑Mouse PTPN22 / PEP Antibody (IHC, WB) LS‑C662657

Antibody:
PTPN22 / PEP Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662657-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PTPN22 / PEP Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PEP antibody LS-C662657 is an unconjugated rabbit polyclonal antibody to PEP (PTPN22) from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Mouse PTPN22 / PEP
Synonyms
PTPN22 | 70zpep | Lymphoid phosphatase | LYP | PEST-domain phosphatase | LYP2 | LYP1 | PEP | PTPN8
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived mouse PTPN22 recombinant protein (Position: M1-Q309).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTPN22 / PEP
Q9Y2R2 NM_015967 NP_057051.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/25/2024