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Catalog Number Size Price
LS-C746996-20 20 µl $287 
LS-C746996-100 100 µl $431 
PTEN Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PTEN antibody at dilution of 1:100 (40x lens).
PTEN Antibody - Immunohistochemistry of paraffin-embedded human stomach using PTEN antibody at dilution of 1:100 (40x lens).
PTEN Antibody - Immunohistochemistry of paraffin-embedded rat heart using PTEN antibody at dilution of 1:100 (40x lens).
PTEN Antibody - Immunofluorescence analysis of U2OS cells using PTEN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
PTEN Antibody - Western blot analysis of extracts of various cell lines, using PTEN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
PTEN Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PTEN antibody at dilution of 1:100 (40x lens).
PTEN Antibody - Immunohistochemistry of paraffin-embedded human stomach using PTEN antibody at dilution of 1:100 (40x lens).
PTEN Antibody - Immunohistochemistry of paraffin-embedded rat heart using PTEN antibody at dilution of 1:100 (40x lens).
PTEN Antibody - Immunofluorescence analysis of U2OS cells using PTEN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
PTEN Antibody - Western blot analysis of extracts of various cell lines, using PTEN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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Polyclonal Rabbit anti‑Human PTEN Antibody (IHC, IF, WB) LS‑C746996

Polyclonal Rabbit anti‑Human PTEN Antibody (IHC, IF, WB) LS‑C746996

Antibody:
PTEN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C746996-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PTEN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PTEN antibody LS-C746996 is an unconjugated rabbit polyclonal antibody to PTEN from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC and WB.
Target
Human PTEN
Synonyms
PTEN | 10q23del | BZS | DEC | GLM2 | MHAM | Phosphatase and tensin homolog | PTEN1 | MMAC1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PTEN (NP_000305.3). MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLEL
Applications
  • IHC (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 19kDa/47kDa/64kDa, while the observed MW by Western blot was 53kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTEN
P60484 NM_000314 NP_000305.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024