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Catalog Number Size Price
LS-C820428-100 100 µl $412 
PSMB8 / LMP7 Antibody - Immunochemical staining of human PSMB8 in human lymphoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
PSMB8 / LMP7 Antibody - Immunofluorescence staining of PSMB8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PSMB8 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
PSMB8 / LMP7 Antibody - Anti-PSMB8 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 25 kDa.
PSMB8 / LMP7 Antibody - PSMB8 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. 2 uL anti-PSMB8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PSMB8 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.
PSMB8 / LMP7 Antibody - Immunochemical staining of human PSMB8 in human lymphoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
PSMB8 / LMP7 Antibody - Immunofluorescence staining of PSMB8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PSMB8 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
PSMB8 / LMP7 Antibody - Anti-PSMB8 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 25 kDa.
PSMB8 / LMP7 Antibody - PSMB8 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. 2 uL anti-PSMB8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PSMB8 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.
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Polyclonal Rabbit anti‑Human PSMB8 / LMP7 Antibody (Preservative Free, IHC, IF, WB) LS‑C820428

Polyclonal Rabbit anti‑Human PSMB8 / LMP7 Antibody (Preservative Free, IHC, IF, WB) LS‑C820428

Antibody:
PSMB8 / LMP7 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C820428-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PSMB8 / LMP7 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
LMP7 antibody LS-C820428 is an unconjugated rabbit polyclonal antibody to human LMP7 (PSMB8). Validated for ICC, IF, IHC, IP and WB.
Target
Human PSMB8 / LMP7
Synonyms
PSMB8 | D6S216 | ALDD | Low molecular mass protein 7 | LMP7 | JMP | Proteasome subunit beta 5i | Proteasome subunit beta type-8 | PSMB5i | Macropain subunit C13 | Y2 | NKJO | Protease component C13 | Proteasome component C13 | Proteasome subunit beta-5i | Proteasome-related gene 7 | RING10 | Beta5i | D6S216E | Low molecular weight protein 7 | Proteasome subunit Y2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
A synthetic peptide corresponding to the C-Terminus of Human PSMB8
Specificity
Human PSMB8
Applications
  • IHC - Paraffin (1:500 - 1:2000)
  • ICC (1:300 - 1:10000)
  • Immunofluorescence (1:300 - 1:10000)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PSMB8 / LMP7
P28062 NM_004159 NP_004150.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/28/2024