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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSMB1 Antibody (aa214‑241) LS‑C162227

Catalog Number Size Price
LS-C162227-400 400 µl $325 
Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMB1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of PSMB1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot of PSMB1 Antibody in mouse NIH-3T3 cell line(lane 1) and mouse bladder tissue(lane 2) lysates (35 ug/lane). PSMB1 (arrow) was detected using the purified antibody.
PSMB1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMB1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of PSMB1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot of PSMB1 Antibody in mouse NIH-3T3 cell line(lane 1) and mouse bladder tissue(lane 2) lysates (35 ug/lane). PSMB1 (arrow) was detected using the purified antibody.
PSMB1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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PSMB1 Antibody (aa214‑241) LS‑C162227

Antibody:
Rabbit Polyclonal to Human PSMB1
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C162227-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PSMB1
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PSMB1 antibody LS-C162227 is an unconjugated rabbit polyclonal antibody to PSMB1 from human and mouse. Validated for Flow, IF, IHC and WB.
Target
Human PSMB1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9817 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C162227. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa214-241
Specificity
This PSMB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-241 amino acids from the C-terminal region of human PSMB1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PSMB1
P20618 NM_002793 NP_002784.1

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Immunohistochemical analysis of PSMB1 staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Product
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Product
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA
Immunohistochemistry of paraffin-embedded mouse brain using PSMB1 antibodyat dilution of 1:100 (40x lens).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Reactivity: Human
Range: 23.5-1500 pg/ml

Requested From: United States
Date Requested: 8/18/2019
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