Human PRSS3 / Trypsin 3

Rat

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived recombinant human rhTrypsin-3.

Recognizes both pro and mature forms of human trypsin 3. Species sequence homology: chimpanzee: 96%, rat: 76%, mouse: 74%, dog: 72%. and chicken: 67%. Does not cross-react with rhTrypsin-1 or rhTrypsin-2.

• Western blot (1 - 2 µg/ml)
• Neutralization

Suitable for use in Neutralization and Western Blot. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.15 ug/ml (IC 50) under conditions in which the enzyme was present at 0.03 ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme. Western blot:1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhTrypsin-3 is ~25 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Reducing conditions are not recommended.

Lyophilized from PBS, 5% Trehalose

Sterile buffer.

Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P35030 NCBI: NM_002771 NP_002762.2