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Antibodies > PRKDC / DNA-PKcs > LS-C810036
 
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Catalog Number Size Price
LS-C810036-100 100 µl (1 mg/ml) $379 
LS-C810036-200 200 µl (1 mg/ml) $421 
PRKDC / DNA-PKcs Antibody - 1:100 staining human colon tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKDC / DNA-PKcs Antibody - Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
PRKDC / DNA-PKcs Antibody - Western blot analysis on HeLa cell lysates using DNA-PK antibody. The lane on the left is treated with the antigen-specific peptide.
PRKDC / DNA-PKcs Antibody - 1:100 staining human colon tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKDC / DNA-PKcs Antibody - Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
PRKDC / DNA-PKcs Antibody - Western blot analysis on HeLa cell lysates using DNA-PK antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human PRKDC / DNA‑PKcs Antibody (IHC, IF, WB) LS‑C810036

Polyclonal Rabbit anti‑Human PRKDC / DNA‑PKcs Antibody (IHC, IF, WB) LS‑C810036

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Antibody:
PRKDC / DNA-PKcs Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C810036-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PRKDC / DNA-PKcs Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
DNA-PKcs antibody LS-C810036 is an unconjugated rabbit polyclonal antibody to DNA-PKcs (PRKDC) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PRKDC / DNA-PKcs
Synonyms
PRKDC | DNA-PK | DNAPK | DNPK1 | HYRC | HYRC1 | p350 | Scid | XRCC7 | DNA-PK catalytic subunit | DNA-PKcs | p460 | DNA-dependent protein kinase catalytic subunit
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human DNA-PK.
Specificity
DNA-PK Antibody detects endogenous levels of DNA-PK.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKDC / DNA-PKcs
P78527 NM_006904 NP_008835.5

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Featured Products

PRKDC / DNA-PKcs Antibody - Anti-DNA-PKcs antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ PRKDC / DNA‑PKcs Antibody (phospho‑Thr2609) LS‑B409
Species: Human, Chimpanzee
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
PRKDC / DNA-PKcs Antibody - Anti-DNA-PKcs antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC‑plus™ PRKDC / DNA‑PKcs Antibody (aa4061‑4110) LS‑B6857
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
PRKDC / DNA-PKcs Antibody - Immunohistochemical analysis of DNA-PKcs (pT2647) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PRKDC / DNA‑PKcs Antibody (phospho‑Thr2647) LS‑C368485
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Product
PRKDC / DNA‑PKcs Antibody (phospho‑Thr2609, clone 10B1) LS‑C41044
Species: Human
Applications: ICC, Immunofluorescence
PRKDC / DNA-PKcs Antibody - Immunohistochemical analysis of DNA-PKcs staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PRKDC / DNA‑PKcs Antibody (C‑Terminus) LS‑C352726
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
PRKDC / DNA-PKcs Antibody - Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse monoclonal antibody (1:200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
PRKDC / DNA‑PKcs Antibody LS‑C393583
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
See all PRKDC / DNA-PKcs Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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