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Catalog Number Size Price
LS-C750604-20 20 µl $287 
LS-C750604-100 100 µl $431 
PRKCQ / PKC-Theta Antibody - Western blot analysis of extracts of Jurkat and HeLa cells, using Phospho-PRKCQ-T538 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 30s.

Polyclonal Rabbit anti‑Human PRKCQ / PKC‑Theta Antibody (phospho‑Thr538, WB) LS‑C750604

Polyclonal Rabbit anti‑Human PRKCQ / PKC‑Theta Antibody (phospho‑Thr538, WB) LS‑C750604

Antibody:
PRKCQ / PKC-Theta Rabbit anti-Human Polyclonal (pThr538) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C750604-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PRKCQ / PKC-Theta Rabbit anti-Human Polyclonal (pThr538) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PKC-Theta antibody LS-C750604 is an unconjugated rabbit polyclonal antibody to human PKC-Theta (PRKCQ) (pThr538). Validated for WB.
Target
Human PRKCQ / PKC-Theta
Synonyms
PRKCQ | NPKC-theta | PKC theta | PRKCT | Protein kinase C theta type | Protein kinase c theta | PKCtheta | Protein kinase C, theta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pThr538
Applications
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 67kDa/74kDa/81kDa, while the observed MW by Western blot was 98kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCQ / PKC-Theta
Q04759 NM_006257 NP_006248.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024