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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PRKACA Antibody (C‑Terminus, clone EP2102Y) IHC‑plus™ LS‑B9373 Best Seller
Note: This antibody replaces LS-C50063
PRKACA antibody LS-B9373 is an unconjugated rabbit monoclonal antibody to PRKACA from human, mouse and rat. Validated for Flow, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9373-50
50 µl (0.617 mg/ml)
$425
Description
PRKACA antibody LS-B9373 is an unconjugated rabbit monoclonal antibody to PRKACA from human, mouse and rat. Validated for Flow, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PRKACA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [EP2102Y]
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:250)
  • ICC (1:100 - 1:250)
  • Western blot (1:50000 - 1:200000)
  • Immunoprecipitation
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PRKACA antibody was raised against a synthetic peptide corresponding to residues near the C-terminus of human PKC C-alpha subunit.
Epitope
C-Terminus
Presentation
Tris-Glycine-NaCl or PBS, pH 7.2-7.4, 40-50% Glycerol, 0.01% sodium azide, 0.05% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PRKACA
P17612 NM_002730 NP_002721.1

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Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, IHC - Paraffin, Western blot
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Images

Immunohistochemistry

Anti-PRKACA antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PRKACA antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot analysis on (A) HeLa and (B) MCF-7 cell lysates using anti- PKA C-alpha antibody, dilution 1:200000.
Western blot analysis on (A) HeLa and (B) MCF-7 cell lysates using anti- PKA C-alpha antibody, dilution 1:200000.

Immunohistochemistry

Anti-PRKACA antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PRKACA antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot analysis on (A) HeLa and (B) MCF-7 cell lysates using anti- PKA C-alpha antibody, dilution 1:200000.
Western blot analysis on (A) HeLa and (B) MCF-7 cell lysates using anti- PKA C-alpha antibody, dilution 1:200000.

Immunohistochemistry

Anti-PRKACA antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PRKACA antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot analysis on (A) HeLa and (B) MCF-7 cell lysates using anti- PKA C-alpha antibody, dilution 1:200000.
Western blot analysis on (A) HeLa and (B) MCF-7 cell lysates using anti- PKA C-alpha antibody, dilution 1:200000.

Immunohistochemistry

Anti-PRKACA antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PRKACA antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot analysis on (A) HeLa and (B) MCF-7 cell lysates using anti- PKA C-alpha antibody, dilution 1:200000.
Western blot analysis on (A) HeLa and (B) MCF-7 cell lysates using anti- PKA C-alpha antibody, dilution 1:200000.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Requested From: United States
Date Requested: 11/21/2018
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