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| Catalog Number | Size | Price |
|---|---|---|
| LS-C745430-100 | 100 µg (0.99 mg/ml) | $567 |
![PRB1 Antibody - Western Blot of rabbit anti-Retinoblastoma [Monomethyl Lys860] antibody. Lane 1: MW ladder (opal pre-stained). Lane 2: MOLT-4 WCL. Load: 35 ug per lane. Primary antibody: Retinoblastoma antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody at 1:70,000 for 30 min at RT. Block: BlockOut 30 min at RT. Predicted/Observed size: expect 106 kDa for Retinoblastoma protein.](https://lsbio-7d62.kxcdn.com/image2/prb1-antibody-me-lys860-ls-c745430/445893_3396425.jpg)
![PRB1 Antibody - Dot Blot of rabbit Anti-Retinoblastoma [Monomethyl Lys860] Antibody. Lane 1: Unmodified peptide Retinoblastoma peptide. Lane 2: Retinoblastoma [Monomethyl Lys860] peptide. Load: 1.0, 0.5, and 0.25 picomoles of peptide. Primary antibody: Retinoblastoma [Monomethyl Lys860] antibody at 1.0, 0.5, and 0.25 ug/ml for o/n at 4°C. Secondary antibody: Gt-A-Rb IgG HRP Conjugated Secondary Antibody 611-103-122 @1:40,000 in BLOTTO Blocking Buffer 30min @ RT. Block: 5% BLOTTO 30 min at RT.](https://lsbio-7d62.kxcdn.com/image2/prb1-antibody-me-lys860-ls-c745430/445894_3396426.jpg)
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Polyclonal Rabbit anti‑Human PRB1 Antibody (Me‑Lys860, IHC, WB) LS‑C745430
Polyclonal Rabbit anti‑Human PRB1 Antibody (Me‑Lys860, IHC, WB) LS‑C745430
Antibody:
PRB1 Rabbit anti-Human Polyclonal (Me-Lys860) Antibody
Application:
IHC, WB, IP, ELISA, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PRB1 Rabbit anti-Human Polyclonal (Me-Lys860) Antibody
Application:
IHC, WB, IP, ELISA, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PRB1 antibody LS-C745430 is an unconjugated rabbit polyclonal antibody to human PRB1 (Me-Lys860). Validated for DB, ELISA, IHC, IP and WB.
Target
Human PRB1
Synonyms
PRB1 | Salivary proline-rich protein | Proline-rich peptide II-2 | Basic peptide IB-6 | Peptide P-H | PRB1M | PRB1L
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-Retinoblastoma [Monomethyl Lys860] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a methylated and acetylated synthetic peptide surrounding lysine 860 of human RB1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
Me-Lys860
Specificity
This antibody reacts with human RB1 surrounding aa860 and is specific for K860 Me1. Blast analysis of the sequence of the immunogen shows 100% identity with human and 92% identity to rat and mouse.
Applications
- IHC
- Western blot (1 µg/ml)
- Immunoprecipitation
- ELISA (1:10000 - 1:50000)
- Dot Blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PRB1
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Reactivity:
Mouse
Range:
31.2-2000 pg/ml
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Date Requested: 4/27/2024
