Target
Human PPP2R1A
Synonyms
PPP2R1A | PP2A-Aalpha | PP2AAALPHA | PR65A
Reactivity
Human, Monkey, Mouse, Rat, Drosophila
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide (KLH-coupled) corresponding to the C-Terminus of human PP2A A subunit.
Specificity
Recognizes endogenous levels of human Protein Phosphatase 2A, A Subunit, both a and b isoforms at ~62kD. Does not crossreact with other Protein Phosphatase 2A subunits.
Applications
- Immunofluorescence
- Western blot (1:1000)
- Flow Cytometry (1:50)
Usage
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% w/v BSA, 1X. TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:100. Flow Cytometry: 1:50.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee