Target
Human PPP1R13L / iASPP
Synonyms
PPP1R13L | IASPP | Inhibitor of ASPP protein | NFkB-interacting protein 1 | NKIP1 | RAI4 | RAI | Retinoic acid induced 4 | NFkB interacting protein 1 | PPP1R13BL | Protein iASPP | RelA-associated inhibitor | PPP1R13B-like protein
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of human iASPP (isoform 1) protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with iASPP proteins from human, dog and mouse sources. Expect reactivity against both isoform 1 and isoform 2. Partial reactivity may occur against iASPP from bovine. Reactivity against homologues from other sources is not known.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:1000 - 1:5000)
- ELISA (1:40000 - 1:160000)
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Usage
Immunohistochemistry: LS-B408 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B408 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee