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Antibodies > PPIB / Cyclophilin B > LS-C757732
 
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Catalog Number Size Price
LS-C757732-200 200 µl $393 
PPIB / Cyclophilin B Antibody - Cyclophilin B Antibody staining Cyclophilin B in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
PPIB / Cyclophilin B Antibody - All lanes: Anti-Cyclophilin B Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: BA/F3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PPIB / Cyclophilin B Antibody - All lanes: Anti-Cyclophilin B Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: BA/F3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PPIB / Cyclophilin B Antibody - Overlay histogram showing Hela cells stained with Cyclophilin B Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (Cyclophilin B Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
PPIB / Cyclophilin B Antibody - Cyclophilin B Antibody staining Cyclophilin B in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
PPIB / Cyclophilin B Antibody - All lanes: Anti-Cyclophilin B Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: BA/F3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PPIB / Cyclophilin B Antibody - All lanes: Anti-Cyclophilin B Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: BA/F3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PPIB / Cyclophilin B Antibody - Overlay histogram showing Hela cells stained with Cyclophilin B Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (Cyclophilin B Antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Polyclonal Rabbit anti‑Human PPIB / Cyclophilin B Antibody (IHC, WB) LS‑C757732

Polyclonal Rabbit anti‑Human PPIB / Cyclophilin B Antibody (IHC, WB) LS‑C757732

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Antibody:
PPIB / Cyclophilin B Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757732-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PPIB / Cyclophilin B Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclophilin B antibody LS-C757732 is an unconjugated rabbit polyclonal antibody to Cyclophilin B (PPIB) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
Target
Human PPIB / Cyclophilin B
Synonyms
PPIB | Cyclophilin B | CYP-S1 | S-cyclophilin | Rotamase B | Cyclophilin-like protein | CYPB | OI9 | PPIase B | SCYLP
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This Cyclophilin B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 161-195 amino acids from the human region of human Cyclophilin B.
Applications
  • IHC - Paraffin (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 23.7 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPIB / Cyclophilin B
P23284 NM_000942 NP_000933.1

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PPIB / Cyclophilin B Antibody (aa36‑85, HRP) LS‑C446458
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications: Western blot
PPIB / Cyclophilin B ELISA Kit
Human PPIB / Cyclophilin B (Sandwich ELISA) ELISA Kit - LS-F4871
Reactivity: Human
Range: 78.13-5000 pg/ml
PPIB / Cyclophilin B Protein - Recombinant Cyclophilin B By SDS-PAGE
Rat PPIB / Cyclophilin B Protein (Recombinant His) (aa34-216) - LS-G11832
Source: E. coli
Tag: His
SMAD3 Antibody - Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ SMAD3 Antibody (phospho‑Ser423/425) LS‑B64
Species: Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus laevis, Xenopus tropicalis
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
PYCARD / ASC / TMS1 ELISA Kit
Mouse PYCARD / ASC / TMS1 (Sandwich ELISA) ELISA Kit - LS-F54634
Reactivity: Mouse
Range: 7.81-500 pg/ml
See all PPIB / Cyclophilin B Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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