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Catalog Number Size Price
LS-C817019-100 100 µl $412 
PPHLN1 Antibody - Immunofluorescence staining of PPHLN1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PPHLN1 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
PPHLN1 Antibody - Anti-PPHLN1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa.
PPHLN1 Antibody - PPHLN1 was immunoprecipitated using: Lane A: 0.5 mg 293 Whole Cell Lysate. 1 uL anti-PPHLN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PPHLN1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 55 kDa.
PPHLN1 Antibody - Immunofluorescence staining of PPHLN1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PPHLN1 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
PPHLN1 Antibody - Anti-PPHLN1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa.
PPHLN1 Antibody - PPHLN1 was immunoprecipitated using: Lane A: 0.5 mg 293 Whole Cell Lysate. 1 uL anti-PPHLN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PPHLN1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 55 kDa.
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Polyclonal Rabbit anti‑Human PPHLN1 Antibody (IF, WB) LS‑C817019

Polyclonal Rabbit anti‑Human PPHLN1 Antibody (IF, WB) LS‑C817019

Antibody:
PPHLN1 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$412
LS-C817019-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PPHLN1 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PPHLN1 antibody LS-C817019 is an unconjugated rabbit polyclonal antibody to human PPHLN1. Validated for ICC, IF, IP and WB.
Target
Human PPHLN1
Synonyms
PPHLN1 | Gastric cancer antigen Ga50 | HSPC232 | HSPC206 | Periphilin 1 | Periphilin-1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human PPHLN1 fragment
Specificity
Human PPHLN1
Applications
  • ICC (1:500 - 1:2500)
  • Immunofluorescence (1:500 - 1:2500)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPHLN1
Q8NEY8 NM_016488 NP_057572.5

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024