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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POU4F1 / BRN3A Antibody (clone 7B4) LS‑C197953
BRN3A antibody LS-C197953 is an unconjugated mouse monoclonal antibody to human BRN3A (POU4F1). Validated for ELISA and WB.
Catalog
Size
Price
LS-C197953-100
100 µg
$355
Description
BRN3A antibody LS-C197953 is an unconjugated mouse monoclonal antibody to human BRN3A (POU4F1). Validated for ELISA and WB.
Target
Human POU4F1 / BRN3A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [7B4]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
POU4F1 / BRN3A antibody was raised against pOU4F1 (NP_006228.2, aa331-420 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human POU4F1 / RDC1 / BRN3A
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About POU4F1 / BRN3A
Q01851 NM_006237 NP_006228.3

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Images

Western blot

Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.Lane 1: POU4F1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.Lane 1: POU4F1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.Lane 1: POU4F1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.Lane 1: POU4F1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.Lane 1: POU4F1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.Lane 1: POU4F1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.Lane 1: POU4F1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.Lane 1: POU4F1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 11/21/2018
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