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| Catalog Number | Size | Price |
|---|---|---|
| LS-C9654-200 | 200 µg | $832 |
Monoclonal Mouse anti‑Mouse PML Antibody (aa1‑581, WB) LS‑C9654
Monoclonal Mouse anti‑Mouse PML Antibody (aa1‑581, WB) LS‑C9654
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
PML antibody LS-C9654 is an unconjugated mouse monoclonal antibody to mouse PML (aa1-581). Validated for ICC and WB.
Target
Mouse PML
Synonyms
PML | PP8675 | Promyelocytic leukemia | Protein PML | TRIM19 | Promyelocytic leukemia protein | RING finger protein 71 | MYL | RNF71
Host
Mouse
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Purification
Protein G purified
Modifications
Unmodified
Immunogen
6His fusion protein corresponding to aa 1-581 of mouse PML (promyelocytic leukemia protein).
Epitope
aa1-581
Specificity
Recognizes mouse PML, Mr ~90-106kD, depending on degree of protein modification. Does not cross-react with human. MEF1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with This antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Applications
- ICC
- Western blot (0.5 - 1 µg/ml)
Usage
Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 0.5-1 ug/ml of this lot detected PML in RIPA lysates from mouse embryo fibroblast (MEF1) cells. Immunocytochemistry: An independent laboratory showed positive immunostaining for PML in MEF1 cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PML
Publications (0)
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
![PML Antibody - Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/pml-antibody-clone-1d12-ls-c197930/146085_1128523.jpg)
Species:
Human, Rat
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Source:
Human
Tag:
Myc-DDK (Flag)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
Date Requested: 4/29/2024
