PLK-1 antibody LS-C9649 is an unconjugated mouse monoclonal antibody to human PLK-1 (PLK1). Validated for ELISA, IF, IP and WB.
Human PLK1 / PLK-1
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (1 - 3 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
PLK1 / PLK-1 antibody was raised against full-length human Plk1 (polo-like kinase 1), which shares 93-94% amino acid sequence homology with mouse and rat Plk1.
Specific for human Plk1. On Western blots, it identifies a band at ~63kD. Reactivity has been confirmed with human HeLa S3 and U-2 OS cells by Western blotting and immunofluorescence, with human HeLa S3 and HEK293 cells by immunoprecipitation, and with mouse NIH3T3 and rat NRK cells by immunofluorescence and Western blotting.
Suitable for use in ELISA, Immunoprecipitation, Immunofluorescence and Western Blot. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5-10 ug/test. Immunofluorescence: 0.5-2 ug/test. Western Blot: 1-3 ug/ml.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.