Target
Human PLK1 / PLK-1
Synonyms
PLK1 | Polo like kinase | Polo-like kinase (Drosophila) | Polo-like kinase 1 | STPK13 | Polo (Drosophia)-like kinase | PLK | PLK-1
Reactivity
Human, Mouse, Rat, Xenopus
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k + IgG2b,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
A His-tagged Plk fusion protein (C-term Plk1), aa 402-603 of the human Plk1 protein.
Specificity
Recognizes the human ~68kD Plk1 protein. Cross-reactivity with a 110kD protein of unknown identity is sometimes observed. Species cross-reactivity: mouse, rat and Xenopus.
Applications
- Immunofluorescence (2 - 5 µg/ml)
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation
- ELISA (0.1 - 1 µg/ml)
Usage
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Immunofluorescence: 2-5 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-2 ug/ml. Immunoprecipitation: 5 ug. Positive control: HeLa, A431 and NIH 3T3 cells.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 40% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee