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Catalog Number Size Price
LS-C745414-100 100 µg (1 mg/ml) $567 
PKM / Pyruvate Kinase, Muscle Antibody - Western Blot of rabbit Anti-PKM2 antibody. Lane 1 and 2: iPSC lysate. Lane 3 and 4: myeloid lysate (iPSC derived). Load: 100 µg per lane. Primary antibody: PKM2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Odyssey blocking buffer for 120 min at RT. Predicted/Observed size: 57 kda for PKM2. Other band(s): PKM2 splice variants and isoforms as PKM2 is a highly modified protein.

Polyclonal Rabbit anti‑Human PKM / Pyruvate Kinase, Muscle Antibody (Internal, WB) LS‑C745414

Polyclonal Rabbit anti‑Human PKM / Pyruvate Kinase, Muscle Antibody (Internal, WB) LS‑C745414

Antibody:
PKM / Pyruvate Kinase, Muscle Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C745414-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PKM / Pyruvate Kinase, Muscle Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Pyruvate Kinase, Muscle antibody LS-C745414 is an unconjugated rabbit polyclonal antibody to human Pyruvate Kinase, Muscle (PKM) (Internal). Validated for ELISA and WB.
Target
Human PKM / Pyruvate Kinase, Muscle
Synonyms
PKM | p58 | PK3 | PKM2 | Pyruvate Kinase | Pyruvate kinase 2/3 | Pyruvate kinase, m1 | Pyruvate kinase, muscle | TCB | Tumor M2-PK | OIP-3 | OIP3 | Opa-interacting protein 3 | CTHBP | PK, muscle type | PK2 | Pyruvate kinase isozymes M1/M2 | Pyruvate kinase muscle isozyme | THBP1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Rabbit, Frog (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.
Epitope
Internal
Specificity
A BLAST analysis was used to suggest reactivity with this protein in African frog and rat based on 100% homology and rabbit and mouse based on 93.3% homology for the immunogen sequence.
Applications
  • Western blot (1 µg/ml)
  • ELISA (5 µg/ml)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PKM / Pyruvate Kinase, Muscle
P14618 NM_002654 NP_002645.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024