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Antibodies > PIK3R1 / p85 Alpha > LS-C808480
 
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Catalog Number Size Price
LS-C808480-100 100 µl (1 mg/ml) $379 
LS-C808480-200 200 µl (1 mg/ml) $421 
PIK3R1 / p85 Alpha Antibody - 1:200 staining human osteosarcoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human skin tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human bladder carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human seminoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human frozen liver tissue section by IHC-Fr. The sample was incubated with the primary antibody (1:200 in BSA) for 1 hour. An Alexa Fluor 488®-conjugated Goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining mouse pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human vascular carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat Intestinal tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human meningeal carcinomatosis(MC) tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human esophageal carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human myosarcoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining mouse lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human liver carcinoma tissues by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human duodenum tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human placenta tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human renal clear cells carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) expression in mouse heart tissue lysate
PIK3R1 / p85 Alpha Antibody - Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) expression in various lysates
PIK3R1 / p85 Alpha Antibody - Western blot analysis of extracts of p-peptide blocked, mouse brain tissue, mouse brain tissue, rat brain tissue and NIH-3T3 cells using Phospho-PI3 kinase p85 alpha (Tyr607) antibody at 1:1000 dilution.; Lane 1 : p-peptide blocked, mouse brain tissue; Lane 2 : mouse brain tissue; lane 3 : NIH-3T3 cells extract; lane 4 : rat brain tissue;
PIK3R1 / p85 Alpha Antibody - Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) antibody expression in H2O2 treated HeLa cells lysates.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human osteosarcoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human skin tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human bladder carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human seminoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human frozen liver tissue section by IHC-Fr. The sample was incubated with the primary antibody (1:200 in BSA) for 1 hour. An Alexa Fluor 488®-conjugated Goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining mouse pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human vascular carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat Intestinal tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining rat gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human meningeal carcinomatosis(MC) tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human esophageal carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human myosarcoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:100 staining mouse lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human liver carcinoma tissues by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human duodenum tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human placenta tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - 1:200 staining human renal clear cells carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PIK3R1 / p85 Alpha Antibody - Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) expression in mouse heart tissue lysate
PIK3R1 / p85 Alpha Antibody - Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) expression in various lysates
PIK3R1 / p85 Alpha Antibody - Western blot analysis of extracts of p-peptide blocked, mouse brain tissue, mouse brain tissue, rat brain tissue and NIH-3T3 cells using Phospho-PI3 kinase p85 alpha (Tyr607) antibody at 1:1000 dilution.; Lane 1 : p-peptide blocked, mouse brain tissue; Lane 2 : mouse brain tissue; lane 3 : NIH-3T3 cells extract; lane 4 : rat brain tissue;
PIK3R1 / p85 Alpha Antibody - Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) antibody expression in H2O2 treated HeLa cells lysates.
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Polyclonal Rabbit anti‑Human PIK3R1 / p85 Alpha Antibody (phospho‑Tyr607, IHC, IF, WB) LS‑C808480

Polyclonal Rabbit anti‑Human PIK3R1 / p85 Alpha Antibody (phospho‑Tyr607, IHC, IF, WB) LS‑C808480

Submit Review LSBio Guarantee
Antibody:
PIK3R1 / p85 Alpha Rabbit anti-Human Polyclonal (pTyr607) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C808480-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PIK3R1 / p85 Alpha Rabbit anti-Human Polyclonal (pTyr607) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
P85 Alpha antibody LS-C808480 is an unconjugated rabbit polyclonal antibody to p85 Alpha (PIK3R1) (pTyr607) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PIK3R1 / p85 Alpha
Synonyms
PIK3R1 | p85 | p85 Alpha | p85-ALPHA | PI3K regulatory subunit alpha | PI3-kinase subunit p85-alpha | GRB1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PI3-kinase p85- alpha around the phosphorylation site of Tyrosine 607.
Epitope
pTyr607
Specificity
Phospho-PI3-kinase p85- alpha (Tyr607) Antibody detects endogenous levels of PI3-kinase p85- alpha only when phosphorylated at Tyrosine 607.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PIK3R1 / p85 Alpha
P27986 M61906 P27986

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See all PIK3R1 / p85 Alpha Antibodies

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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