Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
- Western blot
- Immunoaffinity Column
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Application: Use 10-25 µl of slurry per ml for immunoprecipitation. For affinity chromatography, 1 ml of PY20 agarose is known to bind at least 0.5 mg of tyrosine phosphorylated proteins from RSV-transformed chick embryo fibroblasts. Other applications should be user optimized.
3 mM sodium azide; 50% glycerol, 20 mM sodium phosphate, 150 mM sodium chloride
Store at 4°C to 8°C for up to 1 year. Do not freeze.
For research use only. Intended for use by laboratory professionals.
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