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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PGGT1B Antibody (clone 5E4) LS‑C133375 Best Seller
PGGT1B antibody LS-C133375 is an unconjugated mouse monoclonal antibody to PGGT1B from human and rat. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C133375-100
100 µg
$355

Product Description

PGGT1B antibody LS-C133375 is an unconjugated mouse monoclonal antibody to PGGT1B from human and rat. Validated for ELISA, IF, IHC and WB.

Specifications

Target
Human PGGT1B
Synonyms
PGGT1B, BGGI, GGTI, GGTase-I-beta
Host
Mouse
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [5E4]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1.2 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PGGT1B antibody was raised against pGGT1B (NP_005014, 1 a.a. ~ 106 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human PGGT1B
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About PGGT1B
Protein geranylgeranyltransferase type I (GGTase-I) transfers a geranylgeranyl group to the cysteine residue of candidate proteins containing a C-terminal CAAX motif in which 'A' is an aliphatic amino acid and 'X' is leucine (summarized by Zhang et al., 1994 [PubMed 8106351]). The enzyme is composed of a 48-kD alpha subunit (FNTA; MIM 134635) and a 43-kD beta subunit, encoded by the PGGT1B gene. P53609 NM_005023 NP_005014.2


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Images

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]
Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

PGGT1B monoclonal antibody (M02), clone 5E4 Western blot of PGGT1B expression in HeLa.
PGGT1B monoclonal antibody (M02), clone 5E4 Western blot of PGGT1B expression in HeLa.

Western blot

PGGT1B monoclonal antibody (M02), clone 5E4. Western blot of PGGT1B expression in PC-12.
PGGT1B monoclonal antibody (M02), clone 5E4. Western blot of PGGT1B expression in PC-12.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PGGT1B is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PGGT1B is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]
Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

PGGT1B monoclonal antibody (M02), clone 5E4 Western blot of PGGT1B expression in HeLa.
PGGT1B monoclonal antibody (M02), clone 5E4 Western blot of PGGT1B expression in HeLa.

Western blot

PGGT1B monoclonal antibody (M02), clone 5E4. Western blot of PGGT1B expression in PC-12.
PGGT1B monoclonal antibody (M02), clone 5E4. Western blot of PGGT1B expression in PC-12.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PGGT1B is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PGGT1B is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]
Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

PGGT1B monoclonal antibody (M02), clone 5E4 Western blot of PGGT1B expression in HeLa.
PGGT1B monoclonal antibody (M02), clone 5E4 Western blot of PGGT1B expression in HeLa.

Western blot

PGGT1B monoclonal antibody (M02), clone 5E4. Western blot of PGGT1B expression in PC-12.
PGGT1B monoclonal antibody (M02), clone 5E4. Western blot of PGGT1B expression in PC-12.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PGGT1B is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PGGT1B is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]
Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

PGGT1B monoclonal antibody (M02), clone 5E4 Western blot of PGGT1B expression in HeLa.
PGGT1B monoclonal antibody (M02), clone 5E4 Western blot of PGGT1B expression in HeLa.

Western blot

PGGT1B monoclonal antibody (M02), clone 5E4. Western blot of PGGT1B expression in PC-12.
PGGT1B monoclonal antibody (M02), clone 5E4. Western blot of PGGT1B expression in PC-12.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PGGT1B is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PGGT1B is approximately 1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 11/13/2018

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