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Catalog Number Size Price
LS-C662071-10 10 µg $318 
LS-C662071-100 100 µg $470 
PGF / PLGF Antibody - Western blot analysis of PGF using anti-PGF antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: rat liver tissue lysates,Lane 3: mouse gaster tissue lysates,Lane 4: mouse liver tissue lysates,Lane 5: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGF antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PGF at approximately 25KD. The expected band size for PGF is at 25KD.
PGF / PLGF Antibody - Western blot - Anti-PGF/Plgf Picoband Antibody
PGF / PLGF Antibody - Western blot analysis of PGF using anti-PGF antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: rat liver tissue lysates,Lane 3: mouse gaster tissue lysates,Lane 4: mouse liver tissue lysates,Lane 5: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGF antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PGF at approximately 25KD. The expected band size for PGF is at 25KD.
PGF / PLGF Antibody - Western blot - Anti-PGF/Plgf Picoband Antibody
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Polyclonal Rabbit anti‑Rat PGF / PLGF Antibody (WB) LS‑C662071

Polyclonal Rabbit anti‑Rat PGF / PLGF Antibody (WB) LS‑C662071

Antibody:
PGF / PLGF Rabbit anti-Rat Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662071-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PGF / PLGF Rabbit anti-Rat Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PLGF antibody LS-C662071 is an unconjugated rabbit polyclonal antibody to rat PLGF (PGF). Validated for ELISA and WB.
Target
Rat PGF / PLGF
Synonyms
PGF | D12S1900 | PGFL | PlGF-2 | Placental growth factor | Placental growth factor-like | PLGF | Placenta growth factor | SHGC-10760
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived rat PGF recombinant protein (Position: A24-L158).
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PGF / PLGF
P49763 NM_002632 NP_002623.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/19/2024