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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDX1 Antibody (N‑Terminus, HRP) LS‑C436256
PDX1 antibody LS-C436256 is an HRP-conjugated rabbit polyclonal antibody to PDX1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C436256-100
100 µl (0.65 mg/ml)
$385

Popular PDX1 Products

Anti-PDX1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Monkey, Rabbit
Applications: IHC, IHC - Paraffin, ELISA
Dot blot of anti-Phospho-IPF-pT11 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Species: Human
Applications: Dot Blot
Immunohistochemistry: PDX1 Antibody - IHC analysis of PDX1 in mouse pancreas.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
IHC of paraffin-embedded Human pancreas tissue using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)(1:150).
Species: Human
Applications: IHC, IHC - Paraffin
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Xenopus
Applications: IHC, IHC - Paraffin, Western blot

Product Description

PDX1 antibody LS-C436256 is an HRP-conjugated rabbit polyclonal antibody to PDX1 from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Human PDX1
Synonyms
PDX1, Glucose-sensitive factor, Insulin upstream factor 1, IPF1, Insulin promoter factor 1, IPF-1, IUF-1, STF-1, GSF, IDX-1, Islet/duodenum homeobox-1, IUF1, MODY4, PDX-1
Host
Rabbit
Reactivity
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
PDX1 antibody was raised against synthetic peptide from N-Terminus of human PDX1 (P52945, NP_000200). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Rabbit, Horse, Pig (100%); Galago, Panda, Bat, Opossum (92%).
Epitope
N-Terminus
Specificity
Human PDX1
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About PDX1
Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. P52945 NM_000209 NP_000200.1


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Images

Western blot

PDX1 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PDX1 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PDX1 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PDX1 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PDX1 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PDX1 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PDX1 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PDX1 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/16/2018

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