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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDX1 Antibody (aa100‑200) LS‑C359335
PDX1 antibody LS-C359335 is an unconjugated rabbit polyclonal antibody to PDX1 from human, mouse and rat. Validated for ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C359335-100
100 µl (1 mg/ml)
Unavailable

Popular PDX1 Products

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Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Xenopus
Applications: IHC, IHC - Paraffin, Western blot

Product Description

PDX1 antibody LS-C359335 is an unconjugated rabbit polyclonal antibody to PDX1 from human, mouse and rat. Validated for ICC, IF, IHC and WB.
About PDX1
Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. P52945 NM_000209 NP_000200.1

PDX1 Antibody, Glucose-sensitive factor Antibody, Insulin upstream factor 1 Antibody, IPF1 Antibody, Insulin promoter factor 1 Antibody, IPF-1 Antibody, IUF-1 Antibody, STF-1 Antibody, GSF Antibody, IDX-1 Antibody, Islet/duodenum homeobox-1 Antibody, IUF1 Antibody, MODY4 Antibody, PDX-1 Antibody


Specifications

Target
Human PDX1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • ICC (1:200 - 1:500)
  • Immunofluorescence (1:200 - 1:500)
  • Western blot (1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PDX1 antibody was raised against a synthetic peptide made to a C-terminal portion of the human PDX1 protein (between residues 100-200) [UniProt P52945]
Epitope
aa100-200
Specificity
Human PDX1
Usage
This PDX1 antibody is useful for Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. In Western blot a band is observed ~40 kDa, and in ICC/IF diffuse nuclear and cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Presentation
PBS, 0.02% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Immunohistochemistry: PDX1 Antibody - IHC analysis of PDX1 in mouse pancreas.
Immunohistochemistry: PDX1 Antibody - IHC analysis of PDX1 in mouse pancreas.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: PDX1 Antibody - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunocytochemistry/Immunofluorescence: PDX1 Antibody - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western blot

Western Blot: PDX1 Antibody - WB analysis of PDX1 in INS1 cell lysate.
Western Blot: PDX1 Antibody - WB analysis of PDX1 in INS1 cell lysate.

Immunohistochemistry

Immunohistochemistry: PDX1 Antibody - IHC analysis of PDX1 in mouse pancreas.
Immunohistochemistry: PDX1 Antibody - IHC analysis of PDX1 in mouse pancreas.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: PDX1 Antibody - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunocytochemistry/Immunofluorescence: PDX1 Antibody - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western blot

Western Blot: PDX1 Antibody - WB analysis of PDX1 in INS1 cell lysate.
Western Blot: PDX1 Antibody - WB analysis of PDX1 in INS1 cell lysate.

Immunohistochemistry

Immunohistochemistry: PDX1 Antibody - IHC analysis of PDX1 in mouse pancreas.
Immunohistochemistry: PDX1 Antibody - IHC analysis of PDX1 in mouse pancreas.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: PDX1 Antibody - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunocytochemistry/Immunofluorescence: PDX1 Antibody - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western blot

Western Blot: PDX1 Antibody - WB analysis of PDX1 in INS1 cell lysate.
Western Blot: PDX1 Antibody - WB analysis of PDX1 in INS1 cell lysate.

Immunohistochemistry

Immunohistochemistry: PDX1 Antibody - IHC analysis of PDX1 in mouse pancreas.
Immunohistochemistry: PDX1 Antibody - IHC analysis of PDX1 in mouse pancreas.

Immunofluorescence

Immunocytochemistry/Immunofluorescence: PDX1 Antibody - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunocytochemistry/Immunofluorescence: PDX1 Antibody - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western blot

Western Blot: PDX1 Antibody - WB analysis of PDX1 in INS1 cell lysate.
Western Blot: PDX1 Antibody - WB analysis of PDX1 in INS1 cell lysate.

Requested From: 
Date Requested: 7/15/2018

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