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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDLIM4 / RIL Antibody (aa317‑330) IHC‑plus™ LS‑B9187 Best Seller
Note: This antibody replaces LS-C55255
RIL antibody LS-B9187 is an unconjugated goat polyclonal antibody to RIL (PDLIM4) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9187-50
50 µg (0.5 mg/ml)
$425
Description
RIL antibody LS-B9187 is an unconjugated goat polyclonal antibody to RIL (PDLIM4) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PDLIM4 / RIL
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PDLIM4 / RIL antibody was raised against synthetic peptide C-DVVAVYPNAKVELV from the C-terminus of human PDLIM4 (NP_003678.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Chicken, Armadillo, Xenopus (100%); Galago, Salmon, Stickleback, Zebrafish (93%); Pufferfish (86%).
Blocking Peptide
LS-E25669 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9187. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa317-330
Specificity
Human PDLIM4.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PDLIM4 / RIL
P50479 NM_003687 NP_003678.2

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Positive Western Blot detected in K562 whole cell lysate. All lanes: PDLIM4 antibody at 6.1 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36, 26 KDa. Observed band size: 36 KDa
Species: Human
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Images

Immunohistochemistry

Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry

Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry

Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry

Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PDLIM4 / RIL antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Antibody staining (0.2 ug/ml) of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Requested From: United States
Date Requested: 12/12/2018
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