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Catalog Number Size Price
LS-C818204-100 100 µl $412 
PDI / P4HB Antibody - Immunochemical staining of human P4HB in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
PDI / P4HB Antibody - Immunofluorescence staining of P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human P4HB polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
PDI / P4HB Antibody - Anti-P4HB rabbit polyclonal antibody at 1:1000 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.
PDI / P4HB Antibody - P4HB was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-P4HB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-P4HB rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.
PDI / P4HB Antibody - Immunochemical staining of human P4HB in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
PDI / P4HB Antibody - Immunofluorescence staining of P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human P4HB polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
PDI / P4HB Antibody - Anti-P4HB rabbit polyclonal antibody at 1:1000 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.
PDI / P4HB Antibody - P4HB was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-P4HB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-P4HB rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.
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Polyclonal Rabbit anti‑Human PDI / P4HB Antibody (Preservative Free, IHC, IF, WB) LS‑C818204

Polyclonal Rabbit anti‑Human PDI / P4HB Antibody (Preservative Free, IHC, IF, WB) LS‑C818204

Antibody:
PDI / P4HB Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C818204-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PDI / P4HB Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
P4HB antibody LS-C818204 is an unconjugated rabbit polyclonal antibody to human P4HB (PDI). Validated for ELISA, ICC, IF, IHC, IP and WB.
Target
Human PDI / P4HB
Synonyms
P4HB | ERBA2L | DSI | GIT | p4HB | p4Hbeta | PDIA1 | PHDB | PO4DB | PROHB | Protocollagen hydroxylase | Protein disulfide-isomerase | PO4HB | PDI
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
Recombinant Human P4HB protein
Specificity
Human P4HB.
Applications
  • IHC - Paraffin (1:500 - 1:2000)
  • ICC (1:300 - 1:10000)
  • Immunofluorescence (1:300 - 1:10000)
  • Western blot (1:5000 - 1:10000)
  • Immunoprecipitation
  • ELISA (1:5000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDI / P4HB
P07237 NM_000918 NP_000909.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/30/2024