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Catalog Number	Size	Price
LS-C662065-10	10 µg	$318
LS-C662065-100	100 µg	$470

PDCD4 Antibody - Western blot analysis of PDCD4 using anti-PDCD4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse ovary tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 60KD. The expected band size for PDCD4 is at 52KD.

Polyclonal Rabbit anti‑Human PDCD4 Antibody (WB) LS‑C662065

Name: Anti-PDCD4 Antibody | Rabbit anti-Human Polyclonal WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662065-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

PDCD4 Rabbit anti-Human Polyclonal Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662065-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PDCD4 Rabbit anti-Human Polyclonal Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

PDCD4 antibody LS-C662065 is an unconjugated rabbit polyclonal antibody to human PDCD4. Validated for WB.

Target

Human PDCD4

Synonyms

PDCD4 | H731 | Nuclear antigen H731-like | Nuclear antigen H731 | Protein 197/15a

See All PDCD4 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived human PDCD4 recombinant protein (Position: E346-L446). Human PDCD4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat PDCD4, respectively.

Specificity

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. .

Applications

Western blot

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About PDCD4

Uniprot: Q53EL6 NCBI: NM_014456 NP_055271.2