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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C773200-100 100 µg (1 mg/ml) $395 
PCDHGA / Protocadherin Gamma A Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody, Clone S144-32. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Protocadherin Gamma A (pan) Antibody. (D) Composite.
PCDHGA / Protocadherin Gamma A Antibody - Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A (pan) protein using Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody, Clone S144-32. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
PCDHGA / Protocadherin Gamma A Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody, Clone S144-32. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Protocadherin Gamma A (pan) Antibody. (D) Composite.
PCDHGA / Protocadherin Gamma A Antibody - Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A (pan) protein using Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody, Clone S144-32. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A (pan) Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
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PCDHGA / Protocadherin Gamma A Antibody (clone S144‑32, APC) LS‑C773200

PCDHGA / Protocadherin Gamma A Antibody (clone S144‑32, APC) LS‑C773200

Antibody:
Mouse Monoclonal to Human PCDHGA / Protocadherin Gamma A
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Allophycocyanin, Unmodified
Other formats:
Price
Catalog Number
$395 
LS-C773200-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human PCDHGA / Protocadherin Gamma A
Application:
IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Allophycocyanin, Unmodified
Other formats:

Specifications

Description
Protocadherin Gamma A antibody LS-C773200 is an APC-conjugated mouse monoclonal antibody to Protocadherin Gamma A (PCDHGA ) from human, mouse and rat. Validated for IF and WB.
Target
Human PCDHGA / Protocadherin Gamma A
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
S144-32
Conjugations
Allophycocyanin. Also available conjugated with AP, Atto 390, Atto 488, Atto 565, Atto 594, Atto 633, Atto 655, Atto 680, Atto 700, Biotin, FITC, PE, Atto 594, PerCP, RPE, SA.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Immunogen
Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma-protocadherin-A3.
Specificity
Detects ~100 kDa. Cross-reacts with other Gamma protocadherin-A proteins. No cross-reactivity against Gamma protocadherin-B or -C proteins.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/15/2019