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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C773197-100 100 µg (1 mg/ml) $395 
PCDHG / Protocadherin Gamma Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone S159-5. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Protocadherin Gamma (pan) Antibody. (D) Composite.
PCDHG / Protocadherin Gamma Antibody - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone S159-5. Load: 10 µg. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.
PCDHG / Protocadherin Gamma Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody, Clone S159-5. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma (pan) Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Protocadherin Gamma (pan) Antibody. (D) Composite.
PCDHG / Protocadherin Gamma Antibody - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone S159-5. Load: 10 µg. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.
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PCDHG / Protocadherin Gamma Antibody (clone S159‑5, Atto 700) LS‑C773197

PCDHG / Protocadherin Gamma Antibody (clone S159‑5, Atto 700) LS‑C773197

Antibody:
Mouse Monoclonal to Human PCDHG / Protocadherin Gamma
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Atto 700, Unmodified
Other formats:
Price
Catalog Number
$395 
LS-C773197-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human PCDHG / Protocadherin Gamma
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Atto 700, Unmodified
Other formats:

Specifications

Description
Protocadherin Gamma antibody LS-C773197 is an Atto 700-conjugated mouse monoclonal antibody to Protocadherin Gamma (PCDHG ) from human, mouse and rat. Validated for IF, IHC and WB.
Target
Human PCDHG / Protocadherin Gamma
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
S159-5
Conjugations
Atto 700. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 565, Atto 594, Atto 633, Atto 655, Atto 680, Biotin, FITC, PE, Atto 594, PerCP, RPE, SA.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1
Specificity
Detects ~100 kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/15/2019