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Catalog Number Size Price
LS-C354944-100 100 µg $395 
PAX6 Antibody - Immunohistochemistry analysis of PAX6 showing staining in the nucleus of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- PAX6 Polyclonal Antibody diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
PAX6 Antibody - Immunofluorescence analysis of PAX6 was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PAX6 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
PAX6 Antibody - Immunofluorescent analysis of Pax6 (red) in human neural stem cells derived from PD-3 iPSCs. The cells were fixed and permeabilized, and blocked with the including blocking buffer for one hour at room temperature. Cells were stained with a Pax6 polyclonal antibody at a dilution of 1:100 and a Sox2 monoclonal antibody at a dilution of 1:200 in blocking buffer for 3 hours at room temperature, and then incubated with a DyLight 594-conjugated goat anti-rabbit IgG and a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI. Images were taken at 10X magnification.
PAX6 Antibody - WB using PAX6 Antibody
PAX6 Antibody - Flow cytometry analysis of Pax6 on human neural stem cells derived from PD-3 iPSCs using neural induction medium. Cells were fixed, permeabilized, and then stained with a Pax6 polyclonal antibody at a 1:100 dilution and a Nestin mouse monoclonal antibody at a 1:100 dilution. After incubation of the primary antibodies for 1 hour on ice, the cells were stained with Alexafluor® 488-conjugated goat anti-rabbit IgG secondary antibody and Alexafluor® 647-conjugated donkey anti-mouse IgG secondary antibody at a dilution of 1:500 for 1 hour on ice. A representative 10,000 cells were acquired for each sample.
PAX6 Antibody - Immunohistochemistry analysis of PAX6 showing staining in the nucleus of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- PAX6 Polyclonal Antibody diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
PAX6 Antibody - Immunofluorescence analysis of PAX6 was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PAX6 Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
PAX6 Antibody - Immunofluorescent analysis of Pax6 (red) in human neural stem cells derived from PD-3 iPSCs. The cells were fixed and permeabilized, and blocked with the including blocking buffer for one hour at room temperature. Cells were stained with a Pax6 polyclonal antibody at a dilution of 1:100 and a Sox2 monoclonal antibody at a dilution of 1:200 in blocking buffer for 3 hours at room temperature, and then incubated with a DyLight 594-conjugated goat anti-rabbit IgG and a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI. Images were taken at 10X magnification.
PAX6 Antibody - WB using PAX6 Antibody
PAX6 Antibody - Flow cytometry analysis of Pax6 on human neural stem cells derived from PD-3 iPSCs using neural induction medium. Cells were fixed, permeabilized, and then stained with a Pax6 polyclonal antibody at a 1:100 dilution and a Nestin mouse monoclonal antibody at a 1:100 dilution. After incubation of the primary antibodies for 1 hour on ice, the cells were stained with Alexafluor® 488-conjugated goat anti-rabbit IgG secondary antibody and Alexafluor® 647-conjugated donkey anti-mouse IgG secondary antibody at a dilution of 1:500 for 1 hour on ice. A representative 10,000 cells were acquired for each sample.
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PAX6 Antibody LS‑C354944

PAX6 Antibody LS‑C354944

Antibody:
Rabbit Polyclonal to Human PAX6
Application:
IHC, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$395 
LS-C354944-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PAX6
Application:
IHC, IF, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PAX6 antibody LS-C354944 is an unconjugated rabbit polyclonal antibody to PAX6 from human, mouse and rat. Validated for Flow, IF, IHC and WB.
Target
Human PAX6
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human Pax6.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PAX6
P26367 NM_001604 NP_001595.2

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Requested From: United States
Date Requested: 1/23/2020