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Catalog Number	Size	Price
LS-C815500-100	100 µl	$412

PAK1 Antibody - Immunofluorescence staining of PAK1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-rat PAK1 polyclonal antibody (dilution ratio: 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.

PAK1 Antibody - Anti-PAK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 72 kDa. (We are unsure as to the identity of these extra bands.)

PAK1 Antibody - PAK1 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg NIH-3T3 Whole Cell Lysate. 1 uL anti-PAK1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-PAK1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 66 kDa. Observed band size: 72 kDa.

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Polyclonal Rabbit anti‑Rat PAK1 Antibody (Preservative Free, IF, WB) LS‑C815500

Name: Anti-PAK1 Antibody | Rabbit anti-Rat Polyclonal Preservative Free | LSBio
Brand: Lifespan BioSciences
SKU: LS-C815500-100
Price: 412.0000 USD
Availability: InStock