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Catalog Number Size Price
LS-C413063-100 100 µl (1 mg/ml) $321 
PAK1 Antibody - Immunohistochemical analysis of PAK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PAK1 Antibody - Immunofluorescent analysis of PAK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PAK1 Antibody - Western blot analysis of PAK1 expression in HeLa (A); HEK293T (B) whole cell lysates.
PAK1 Antibody - Immunohistochemical analysis of PAK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PAK1 Antibody - Immunofluorescent analysis of PAK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PAK1 Antibody - Western blot analysis of PAK1 expression in HeLa (A); HEK293T (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human PAK1 Antibody (Internal, IHC, IF, WB) LS‑C413063

Polyclonal Rabbit anti‑Human PAK1 Antibody (Internal, IHC, IF, WB) LS‑C413063

Antibody:
PAK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$321
LS-C413063-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PAK1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PAK1 antibody LS-C413063 is an unconjugated rabbit polyclonal antibody to PAK1 (Internal) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human PAK1
Synonyms
PAK1 | Alpha-PAK | p21-activated kinase 1 | p65-PAK | Rac/p21-activated kinase | STE20 homolog, yeast | PAKalpha | PAK-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human PAK1.
Epitope
Internal
Specificity
Recognizes endogenous levels of PAK1 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PAK1
Q13153 NM_002576 NP_002567.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/17/2024