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Catalog Number Size Price
LS-C808686-100 100 µl (1 mg/ml) $379 
LS-C808686-200 200 µl (1 mg/ml) $421 
PAK1 Antibody - 1/100 staining human colon  tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
PAK1 Antibody - 1:100 staining human liver carcinoma tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PAK1 Antibody
PAK1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PAK1 Antibody - Western blot analysis of PAK1 expression in Etoposide treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PAK1 Antibody - Western blot analysis of PAK1 expression in mouse brain tissue lysates. The lane on the right is treated with the antigen-specific peptide.
PAK1 Antibody - 1/100 staining human colon  tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
PAK1 Antibody - 1:100 staining human liver carcinoma tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PAK1 Antibody
PAK1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PAK1 Antibody - Western blot analysis of PAK1 expression in Etoposide treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PAK1 Antibody - Western blot analysis of PAK1 expression in mouse brain tissue lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human PAK1 Antibody (IHC, IF, WB) LS‑C808686

Polyclonal Rabbit anti‑Human PAK1 Antibody (IHC, IF, WB) LS‑C808686

Antibody:
PAK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C808686-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PAK1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PAK1 antibody LS-C808686 is an unconjugated rabbit polyclonal antibody to PAK1 from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PAK1
Synonyms
PAK1 | Alpha-PAK | p21-activated kinase 1 | p65-PAK | Rac/p21-activated kinase | STE20 homolog, yeast | PAKalpha | PAK-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PAK1.
Specificity
PAK1 Antibody detects endogenous levels of total PAK1.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PAK1
Q13153 NM_002576 NP_002567.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/17/2024